K0254

Kanamycin solution from Streptomyces kanamyceticus

50 - 60 mg/mL in 0.9% NaCl, BioReagent, liquid, 0.1 μm filtered, suitable for cell culture

• Synonym(s): Kanamycin A
• CAS Number: 25389-94-0
• MDL number: MFCD00070289
• UNSPSC Code: 12352207
• PubChem Substance ID: 329817120
• NACRES: NA.76

Properties

• biological source: Streptomyces kanamyceticus
• Quality Level: 200
• sterility: 0.1 μm filtered
• product line: BioReagent
• form: liquid
• concentration: 50 - 60 mg/mL in 0.9% NaCl
• technique(s): cell culture | mammalian: suitable; cell culture | plant: suitable
• impurities: endotoxin, tested
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• Mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17/h4-18,23-29H,1-3,19-22H2/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-/m1/s1
• InChI key: SBUJHOSQTJFQJX-NOAMYHISSA-N

Description

General description

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 2 mL/L.

Application

Kanamycin solution from Streptomyces kanamyceticus has been used:

• to induce deafness in neonatal rats
• as an endothelial growth medium component for culturing human umbilical vein endothelial cells (HUVECs)
• as medium component for Mycobacteria Cultures and Top10 E coli cells

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Caution

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H360D
• Precautionary Statements: P201 - P308 + P313
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable