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GE17-5197-01

HiTrap® Butyl Fast Flow

Cytiva 17-5197-01, pack of 5 × 5 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

butyl

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5197-01

parameter

<20 mL/min flow rate
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium

General description

HiTrap® Butyl FF are prepacked Butyl Sepharose™ 4 Fast FLow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.

Features and Benefits

  • Provide convenient and fast small-scale Protein purifications.
  • Butyl Sepharose™ 4 Fast FLow is a well-established, standard aliphatic HIC medium for capture and intermediate purification.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA® design.
  • High fLow rates and high capacities.
  • Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

Protocols

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service