Skip to Content
Merck
All Photos(1)

Documents

GE17-1014-01

Q Sepharose High Performance

Cytiva 17-1014-01, pack of 75 mL

Synonym(s):

Q Sepharose Resin

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

quaternary amine

description

Ion Exchanger Type (value)

packaging

pack of 75 mL

manufacturer/tradename

Cytiva 17-1014-01

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning in place

1-14

working range

2-12

suitability

suitable for bioprocess medium

Related Categories

General description

Q Sepharose High Performance is a strong anion exchange BioProcess resin with high resolution designed for high intermediate and polishing steps in downstream processing.

Q Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. Q Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide.

Q Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Q Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support.

Application

Ion Exchange Chromatography (IEX) separates molecules on the bases of differences in their net surface charge. Anion Exchange Chromatography (AIEX) medium interacts with negatively charged proteins and are separated by selective salt elution.

Features and Benefits

  • Strong anion exchanger for intermediate and final polishing steps
  • High resolution, high capacity separation with high recovery
  • High chemical stability for effective CIP/sanitization
  • BioProcess resin supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Protocols

Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.

This page discusses how to do a purification with SOURCE™ media from Cytiva.

This page discusses various aspects of sample preparation for chromatographic purification.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service