G0411
Prionex® Highly purified Type A
aqueous solution
Synonym(s):
Gelatin, Prionex® Highly purified Type A
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About This Item
Recommended Products
biological source
Porcine bone
Quality Level
sterility
aseptically processed
form
aqueous solution
technique(s)
ELISA: suitable
microbiological culture: suitable
western blot: suitable
General description
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type-A gelatin is derived from acid-cured (acid-hydrolyzed) tissue. Type-B gelatin is derived from lime-cured (base/alkaline-hydrolyzed) tissue. Gelatin is a biocompatible polymer.
Application
Prionex® Highly purified Type A has been used:
- for coating cell culture to improve attachment of cells, and was added to polymerase chain reaction (PCR) to help stabilize Taq DNA
- as a blocking reagent in Western blotting, enzyme linked immunosorbent assay (ELISA), and immunohistochemistry
- as a component of media for species differentiation in bacteriology
- as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for engineering applications
- in the murine lethality assay which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo
Legal Information
Prionex is a registered trademark of Pentapharm AG, Basel
Storage Class Code
11 - Combustible Solids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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PCR Primer: A Laboratory Manual (1995)
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Journal of immunological methods, 101(1), 43-50 (1987-07-16)
We tested instantized dry milk, casein, gelatins from pig and fish skin, serum albumin and several other proteins for their abilities to block non-specific binding (NSB) of a peroxidase-conjugated immunoglobulin to polystyrene microtiter plate wells. Each blocking protein was tested
Hand asepsis: the efficacy of different soaps in the removal of bacteria from sterile, gloved hands.
Journal of the American Dental Association (1939), 113(2), 291-292 (1986-08-01)
The study showed that washing a gloved hand removed significant amounts of bacteria. If the proper soap or scrub is used, the gloved hand will be free of bacteria. It is suggested that all dental personnel wear gloves to protect
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