E6267
Earle′s Balanced Salts
With sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
EBSS
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About This Item
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Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.2 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
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General description
Earle′s Balanced Salts is a balanced salt solution. It is used as an alternative for conventional simulated body fluids (c-SBF).
Recommended for suspension cultures or whenever cell clumping is a problem.
Application
Earle′s Balanced Salts has been used:
- to induce autophagy in mouse embryonic fibroblasts
- in the preparation of melanotroph isolation medium
- in the preparation of DNAse stock solution
Biochem/physiol Actions
Balanced Salt solution participates in providing cells with water, bulk inorganic ions, and nutrients like carbohydrates essential for normal cell metabolism. It acts as an irrigating, transporting, and diluting fluid to maintain cellular osmotic balance. Balance salt solution helps in maintaining the medium within the physiological pH range (7.2 to 7.6).
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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