MOR/0.4R

96042334

• UNSPSC Code: 41106514

Properties

• biological source: human lung
• growth mode: Semi-adherent aggregates
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human lung adenocarcinoma, drug-resistant

Cell Line Description

MOR/0.4R has been developed from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). This drug-resistant cell line accumulates lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance-associated protein (MRP). The expression of a 190kDa membrane protein associated with the degree of drug resistance has been indicated. Cells grow as easily detaching aggregates

Application

Analysis of acquired drug resistance, MRP studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 7
TPOX: 11
vWA: 15

Culture Medium

RPMI 1640 + 2mM Glutamine + 0.4μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

Other Notes

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