Skip to Content
Merck
All Photos(1)

Key Documents

BW5147.G.1.4.OUA/R.1

85082302, mouse blood, Lymphoblast

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514

product name

BW5147.G.1.4.OUA/R.1, 85082302

biological source

mouse blood

growth mode

Suspension

morphology

Lymphoblast

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

General description

For complete product information, please see BW5147.G.1.4.OUA/R.1

Cell Line Origin

Mouse AKR/J T cell lymphoma

Cell Line Description

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

Application

Fusion partner

Culture Medium

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines at sigmaaldrich.com

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service