Recommended Products
product name
B92, 85042304
biological source
rat nerve
growth mode
Adherent
karyotype
Not specified
morphology
Glial/neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat nervous tissue glial
Cell Line Description
Ethyl nitrosaurea induced tumour cell line. Neuronal and glial cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Morphology significantly affected when grown in serum free media.
Application
Neurobiological studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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