A0481
Anti-Axin1 (C-terminal) antibody produced in rabbit
1.5 mg/mL, affinity isolated antibody
Synonym(s):
Anti-Axin, Anti-Axis Inhibitor 1, Anti-Axis inhibition protein 1
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About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
mol wt
antigen ~130 kDa
species reactivity
rat, mouse
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
1.5 mg/mL
technique(s)
western blot: 0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AXIN1(8312)
mouse ... Axin1(12005)
rat ... Axin1(79257)
General description
Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC),,,.
Rabbit Anti-Axin1 (C-terminal) antibody is specific for rat and mouse axin1 by immunoblotting (∼130 kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide
Rabbit Anti-Axin1 (C-terminal) antibody is specific for rat and mouse axin1 by immunoblotting (∼130 kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide
Immunogen
synthetic peptide corresponding to amino acids 815-832 located near the C-terminal of rat axin1, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in human, mouse, and bovine axin1.
Application
Anti-Axin1 (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1). The antibody has also been used for immunoblotting assays in astrocytoma cell lysates.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lobna Elkhadragy et al.
Frontiers in oncology, 12, 904031-904031 (2022-06-08)
Hepatocellular carcinoma (HCC) is an aggressive disease lacking effective treatment. Animal models of HCC are necessary for preclinical evaluation of the safety and efficacy of novel therapeutics. Large animal models of HCC allow testing image-guided locoregional therapies, which are widely
Axin induces cell death and reduces cell proliferation in astrocytoma by activating the p53 pathway.
Li-Ying Zhang et al.
International journal of oncology, 35(1), 25-32 (2009-06-11)
Astrocytic tumors are the most common brain tumors with various genetic defects. As a tumor suppressor gene, Axin could control cell death and growth. Axin possesses a separate domain that directly interacts with p53 and regulates the activity of p53
Xiaoli Zhang et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(8), 2790-2795 (2011-08-03)
High expression of the oncoprotein Myc has been linked to poor outcome in human tumors. Although MYC gene amplification and translocations have been observed, this can explain Myc overexpression in only a subset of human tumors. Myc expression is in
S Kishida et al.
The Journal of biological chemistry, 273(18), 10823-10826 (1998-06-06)
The regulators of G protein signaling (RGS) domain of Axin, a negative regulator of the Wnt signaling pathway, made a complex with full-length adenomatous polyposis coli (APC) in COS, 293, and L cells but not with truncated APC in SW480
Thomas J F Nieland et al.
PloS one, 9(3), e91744-e91744 (2014-03-19)
The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such
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