61743

N-Lauroylsarcosine sodium salt

BioUltra, for molecular biology, ≥99.0% (HPLC)

• Synonym(s): N-lauroylsarcosine sodium salt, N-Dodecanoyl-N-methylglycine sodium salt, Sarkosyl NL
• Linear Formula: CH₃(CH₂)₁₀CON(CH₃)CH₂COONa
• CAS Number: 137-16-6
• Molecular Weight: 293.38
• Beilstein: 5322974
• EC Number: 205-281-5
• MDL number: MFCD00042728
• UNSPSC Code: 12161900
• PubChem Substance ID: 57651862
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• description: anionic
• product line: BioUltra
• Assay: ≥99.0% (HPLC)
• form: powder
• mol wt: micellar avg mol wt 600; average mol wt 600
• aggregation number: 2
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• loss: ≤1% loss on drying, 110 °C
• pH: 7.0-9.0 (25 °C, 1 M in H₂O)
• CMC: 14.6 mM (20-25°C)
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.2; λ: 280 nm A_max: 0.06
• SMILES string: [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O
• application(s): sample preservation
• InChI: 1S/C15H29NO3.Na/c1-3-4-5-6-7-8-9-10-11-12-14(17)16(2)13-15(18)19;/h3-13H2,1-2H3,(H,18,19);/q;+1/p-1
• InChI key: KSAVQLQVUXSOCR-UHFFFAOYSA-M

Description

General description

The N-lauroylsarcosine sodium salt is applicable for processes that require tight control of elemental content. This product aids in the solubilization and separation of membrane proteins, glycoproteins, and the isolation of RNA and plasmids. N-lauroylsarcosine sodium salt serves as an antifoaming supplement or as a component of a lysing buffer of bacteria or other cells. It indicates the paramagnetic anisotropy sign change in the micelle mesophase. N-lauroylsarcosine sodium salt inhibits hexokinase and the bacterial flora of human saliva/gut at 0.25%. It also exhibits fungistatic activity in aqueous dispersion (1%). N-lauroylsarcosine (LS) is an anionic surfactant with protein denaturant potency that is structurally similar to sodium lauryl sulfate (SLS). It can inactivate herpes simplex virus (HSV) infectivity in vitro. It is used as a microbicide in topical vaginal formulations to block the transmission of HSV, human immunodeficiency virus type 1 (HIV-1), and possibly other pathogens causing sexually transmitted diseases (STDs).

Application

N-Lauroylsarcosine sodium salt has been used as a component:

• of nuclease stop mix, embryo extract buffer to dissociate nonspecifically bound proteins from chromatin
• of proteinase K solution for the heat denaturation of genomic DNA
• of 10% (w/v) sarcosyl/0.5 M ethylenediaminetetraacetic acid (EDTA) solution to prepare EDTA-sarcosyl-proteinase (ESP) buffer
• in the hybridization buffer for in situ hybridization histochemistry
• in the embryo extract buffer to dissociate nonspecifically bound proteins from chromatin
• in proteinase K solution to inactivate the enzymes

Other Notes

Anionic surfactant.

Safety Information

• Pictograms: GHS05,GHS06
• Signal Word: Danger
• Hazard Statements: H315 - H318 - H330
• Precautionary Statements: P260 - P264 - P280 - P302 + P352 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 1
• Flash Point(F): 512.6 °F - closed cup
• Flash Point(C): 267 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves