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Sigma-Aldrich

Sudan Black B Staining System

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.47

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

General description

Intended for the histochemical demonstration of neutrophil granules in blood or bone marrow films.

Principle

Following fixation, blood or bone marrow films are immersed in a buffered Sudan Black B solution. After rinsing, slides are counterstained with Gill hematoxylin, and cells are examined microscopically for the presence of blue-black discrete granulation. Granulocytic precursors demonstrate groups of coarse blue-black deposits. Other hematopoietic cells are typically negative.

Kit Components Only

Product No.
Description
  • Sudan Black B Staining Reagent (3801) 200 mL
  • Hematoxylin Solution, Gill No. 3 (GHS3) 100 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • 3802Glutaraldehyde solution, for in vitro diagnostic use 75 mLSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 Oral - STOT SE 2 - STOT SE 3

Target Organs

Eyes,Central nervous system, Kidney, Respiratory system

Supplementary Hazards

EUH071

Storage Class Code

3 - Flammable liquids

Flash Point(F)

69.1 °F

Flash Point(C)

20.6 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yuelan Hou et al.
Scientific reports, 6, 36853-36853 (2016-11-12)
Emergency granulopoiesis occurs in response to severe microbial infection. However, whether and how other blood components, particularly monocytes/macrophages and their progenitors, including hematopoietic stem/progenitor cells (HSPCs), participate in the process and the underlying molecular mechanisms remain unknown. In this study
F Zhao et al.
Leukemia, 32(1), 149-158 (2017-06-20)
Interferon regulatory factor (IRF)-8 is a critical transcription factor involved in the pathogenesis of myeloid neoplasia. However, the underlying mechanisms in vivo are not well known. Investigation of irf8-mutant zebrafish in this study indicated that Irf8 is evolutionarily conserved as
Ana-Maria Chiorcea-Paquim et al.
Analytical chemistry, 87(12), 6141-6149 (2015-05-12)
The interactions of the Tetrahymena telomeric repeat sequence d(TG4T) and the polyguanylic acid (poly(G)) sequence with the quadruplex-targeting triazole-linked acridine ligand GL15 were investigated using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite and voltammetry at a glassy

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