U1250
Urea
ReagentPlus®, ≥99.5%, pellets
Synonym(s):
Carbamide, Carbonyldiamide
About This Item
Recommended Products
Quality Level
product line
ReagentPlus®
Assay
≥99.5%
form
pellets
mp
132-135 °C (lit.)
solubility
H2O: 8 M
density
1.335 g/mL at 25 °C (lit.)
SMILES string
NC(N)=O
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information
human ... CA1(759) , CA2(760)
rat ... Ppm1a(24666)
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General description
Application
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Articles
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
Protocols
To standardize a procedure for the enzymatic assay of Urease, from Jack Beans
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
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