P5530

Phenol Red sodium salt

powder, BioReagent, suitable for cell culture, suitable for insect cell culture

• Synonym(s): Phenolsulfonephthalein sodium salt
• Empirical Formula (Hill Notation): C₁₉H₁₃NaO₅S
• CAS Number: 34487-61-1
• Molecular Weight: 376.36
• Beilstein: 6260026
• EC Number: 252-057-8
• MDL number: MFCD00066901
• UNSPSC Code: 12171500
• PubChem Substance ID: 24898664
• NACRES: NA.47

Properties

• product line: BioReagent
• Quality Level: 200
• form: powder
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• color: red to very dark red
• visual transition interval: 6.8-8.2, yellow to red
• mp: 285 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• εmax: 31.62 at 423 nm in 0.1 M phosphate buffer
• suitability: suitable for (Suitable for use as a pH indicator)
• application(s): diagnostic assay manufacturing
• storage temp.: room temp
• SMILES string: [Na+].Oc1ccc(cc1)C2(OS(=O)(=O)c3ccccc23)c4ccc([O-])cc4
• InChI: 1S/C19H14O5S.Na/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19;/h1-12,20-21H;/q;+1/p-1
• InChI key: HKHYOKBQJILTEI-UHFFFAOYSA-M

Description

General description

Phenol Red Sodium Salt (PRSS) is a water-soluble anionic fluorescent dye, that possesses an extra Na ion attached with the SO3 group compared to phenol red. It is intended for use in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above.

Application

The sodium salt of phenol red is used widely in culture media to identify changes from neutral to acidic pH values and as a pH marker for cell tissue culture. It is typically used in cell culture media at 11 mg/L. Phenol red in tissue culture media can act as a weak estrogen, especially in human breast cancer cells. PRSS plays a role as an indicator in the process of dying cells or overgrowth of cells in different mediums.

Suitable for use as a pH indicator.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P304 + P340 + P312
• Hazard Classifications: Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves