G6639
Guanidine thiocyanate
≥97% (titration)
Synonym(s):
Guanidinium rhodanide, Guanidinium thiocyanate
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About This Item
Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
Molecular Weight:
118.16
Beilstein:
3563461
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.55
biological source
synthetic
Quality Level
Assay
≥97% (titration)
form
powder or crystals
technique(s)
RNA extraction: suitable
color
white to off-white
mp
120-122 °C (lit.)
solubility
water: 4 M, clear, colorless
anion traces
chloride (Cl-): ≤2.5%
SMILES string
SC#N.NC(N)=N
InChI
1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChI key
ZJYYHGLJYGJLLN-UHFFFAOYSA-N
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Application
Guanidine thiocyanate has been used:
- as a denaturing agent to study its effects on surface activity of globular protein
- for the extraction of total RNA from primary hepatocytes
- as a component of the incubating buffer for extraction of DNA from human bone
- as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
- as a component of lysis solution for extraction of RNA from rat pancreatic tissues
Chaotropic agent and strong denaturant; solubilizes cells.
Biochem/physiol Actions
Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Supplementary Hazards
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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