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Key Documents

B0770

Sigma-Aldrich

Brilliant Blue G

≥80% purity (HPLC), powder

Synonym(s):

Acid blue 90, Coomassie Brilliant Blue G

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About This Item

Empirical Formula (Hill Notation):
C47H48N3NaO7S2
CAS Number:
Molecular Weight:
854.02
Colour Index Number:
42655
Beilstein:
5230822
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Brilliant Blue G, pure

Quality Level

Assay

≥80.0% (HPLC)

form

powder

technique(s)

microbe id | staining: suitable

color

dark violet

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

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Application

Brilliant Blue G has been used for determining the urine protein concentration. It has been used for the protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used as a P2X7R (purinergic receptor) antagonist.

Biochem/physiol Actions

Brilliant Blue G is used for staining protein(s) in polyacrylamide gels. It is also a P2X7 purinergic receptor antagonist. The receptor is mainly involved in neuroinflammatory events. In human SOD1G93A amyotrophic lateral sclerosis mouse, administration of brilliant blue G reduces body weight loss. However, it does not affect survival or motor coordination in transgenic mice.
P2X7 purinergic receptor antagonist.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

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