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85469

Millipore

SOC Broth

suitable for microbiology

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About This Item

UNSPSC Code:
41106204

sterility

sterile (Filtered and Aseptic Handled)

Quality Level

form

powder

composition

glucose, 20 mM
magnesium chloride, 10 mM
magnesium sulfate, 10 mM
potassium chloride, 2.5 mM
sodium chloride, 10 mM
tryptone, 20 g/L
yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

food and beverages
life science and biopharma

microbiology

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

Application

SOC Broth is a sterile solution for the recovery of electroporated Escherichia coli cells.

Preparation Note

Immediately after electroporation add 1 ml sterile SOC Medium to the transformed cells and incubate for 30-60 minutes at 37°C with a moderate shaking .

Other Notes

Transformation of E. coli with plasmids

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Advanced Bacterial Genetics, Cold Spring Harbor (2007)
F.M. Ausubel et al.
Current Protocols in Molecular Biology (1987)
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth

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