63184

5-Maleimido-eosin

for fluorescence, ≥93% (HPLC)

• Synonym(s): EMA, Eosin-5-maleimide
• Empirical Formula (Hill Notation): C₂₄H₉Br₄NO₇
• CAS Number: 150322-02-4
• Molecular Weight: 742.95
• MDL number: MFCD07437936
• UNSPSC Code: 12352207
• PubChem Substance ID: 329759808
• NACRES: NA.32

Properties

• grade: for fluorescence
• Quality Level: 100
• Assay: ≥93% (HPLC)
• fluorescence: λ_ex 524 nm; λ_em 545 nm (after derivatization with 2-mercaptoethanol)
• storage temp.: 2-8°C
• SMILES string: Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1Br
• InChI: 1S/C24H9Br4NO7/c25-13-6-11-21(17(27)19(13)32)35-22-12(7-14(26)20(33)18(22)28)24(11)10-2-1-8(5-9(10)23(34)36-24)29-15(30)3-4-16(29)31/h1-7,32-33H
• InChI key: YRCLWXRIEYQTAV-UHFFFAOYSA-N

Description

General description

5-Maleimido-eosin, also known as EMA or Eosin-5-maleimide, is a fluorescent binding agent. The 5-Maleimido-eosin binding test measures the EMA-labeled cells reduced mean channel fluorescence (MCF) reading.

Application

Eosin-5-maleimide has been used as a fluorescent probe for the human erythrocyte band 3 protein. It is widely used as a diagnostic probe for the laboratory detection of hereditary spherocytosis (HS) by flow cytometry. The eosin-5-maleimide binding test was done to analyze the effect of alectinib (first line of treatment) for anaplastic lymphoma kinase (ALK).

Other Notes

Triplet probe. Measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves