BCIPP-RO

BCIP

99% (HPLC), powder, solubility: in DMF, soluble

• Synonym(s): 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BCIP^® p-toluidine salt, X-phosphate p-toluidine salt
• Empirical Formula (Hill Notation): C₈H₆BrClNO₄P · C₇H₉N
• CAS Number: 6578-06-9
• Molecular Weight: 433.62
• MDL number: MFCD00040636
• UNSPSC Code: 12352204
• PubChem Substance ID: 329774194

Properties

• description: 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt
• Quality Level: 100
• Assay: 99% (HPLC)
• form: powder
• mol wt: M_r 326.44 (BCIP); M_r 433.6 (BCIP toluidine salt)
• packaging: pkg of 1 g (11585002001); pkg of 250 mg (10760994001)
• manufacturer/tradename: Roche
• solubility: DMF: soluble
• storage temp.: 2-8°C
• SMILES string: Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
• InChI key: QEIFSLUFHRCVQL-UHFFFAOYSA-N

Description

General description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Quality

Performance test: incubation with AP

Substrates

Histochemical substrate for alkaline phosphatase.

Analysis Note

Absorption: Absorption maximum in the visible light is not determined.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H312 - H315 - H319 - H335
• Precautionary Statements: P261 - P264 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P337 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): 136.4 °F
• Flash Point(C): 58 °C