PMEF-CFL
EmbryoMax® Primary Mouse Embryonic Fibroblasts
Synonym(s):
CF-1 MEF Feeder Cells, CF-1 MEFs, CF-1 PMEFs, CF1 Mouse Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells
About This Item
Recommended Products
biological source
mouse
Quality Level
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
shipped in
liquid nitrogen
General description
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Cell Line Description
Packaging
Physical form
Storage and Stability
Legal Information
Disclaimer
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Protocols
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
This technical article covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
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