MHA000P2S

m-Heterotrophic Plate Count Broth

2ml ampoule for the recovery of stressed heterotrophic bacteria found in various types of water. Also suitable for water samples with low counts.

• Synonym(s): m-HPC Broth
• UNSPSC Code: 41106225
• eCl@ss: 32160801
• NACRES: NA.74

Properties

• Quality Level: 100
• form: liquid
• shelf life: 12 mo.
• feature: ready-to-use
• packaging: pkg of 2 mL; pack of 50 tests
• technique(s): cell counting: suitable (total viable count (TVC))
• color: clear tan to light amber
• input: sample type cider; sample type beverage(s); sample type cosmetics; sample type bottled water; sample type food(s); sample type beer; sample type wine; sample type soft drink; sample type sport drink
• pH: 7.1±0.2 (25.0 °C)
• application(s): clinical; cosmetics; environmental; food and beverages; industrial qc; microbiology; water monitoring
• compatibility: for use with MF-method; for use with Microfil^®; for use with MicropreSure^®; for use with Milliflex^®
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: nonselective for

Description

General description

Heterotrophs are organisms including bacteria, yeasts and moulds that require an external source of organic carbon for growth. The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live heterotrophic bacteria in water.{14} This test can provide useful information about water quality and supporting data on the significance of coliform test results. Heterotrophic Plate Count Broth can be employed for the determination of bacteria by the membrane filter method. Peptic digest of animal tissue is the source of nutrients for organisms, which are not highly fastidious. Gelatin is utilized by microorganisms through a proteolytic mechanism. The addition of glycerol to the basal medium provides a source of carbon and energy

Application

m-HPC broth is used in the recovery of stressed heterotrophic bacteria found in various types of water. Also suitable for water samples with low counts. Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Packaging

2 mL plastic ampoules

Specifications

Incubation Temperature: 30–35 °C

Incubation Time: 48–72 hrs

Standard Methods for the Examination of Water and Wastewater

Preparation Note

Prior to use, warm media at 30-50 °C until liquefied. Do not microwave.

Culture Medium

m-HPC

Analysis Note

Colonies appear clear to creamy white, some may produce pigment

QC organisms
E. coli (ATCC 25922)

S. epidermidis (ATCC 14990)

Target Microorganisms: Total Count

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable