MABT153

Anti-Aggrecan Antibody

mouse monoclonal, 4D7.1

• Synonym(s): Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• product name: Anti-Aggrecan Antibody (full length), clone 4D7.1, clone 4D7.1, 1 mg/mL, from mouse
• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4D7.1, monoclonal
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_037359
• UniProt accession no.: P16112
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ACAN(176)

Description

General description

Aggrecan is an extracellular protein that is also known as Aggrecan core protein, Cartilage-specific proteoglycan core protein (CSPCP), Chondroitin sulfate proteoglycan core protein 1, and Chondroitin sulfate proteoglycan 1. Aggrecan is encoded by the gene ACAN/AGC1/CSPG1/MSK16. Aggrecan is a proteoglycan that is a major component of the extracellular matrix of cartilaginous tissues. The major function of Aggrecan is to resist compression in cartilage, thus providing protection for joint movement and against injury. Aggrecan binds various other ECM proteins via its large globular domains. Aggrecan can be cleaved into Aggrecan core protein 2, a C-terminal fragment component that decreases in cartilage as people age. Aggrecan is expressed in cartilage and is a massive (>2500 kDa) proteoglycan protein that has multiple isoforms. The appearance of Aggrecan fragments in the blood is a marker for various joint diseases.

Immunogen

KLH-conjugated linear peptide corresponding to human Aggrecan.

Application

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Aggrecan Antibody (full length), clone 4D7.1 is validated for use in Western Blotting for the detection of Aggrecan (full length).

Quality

Evaluated by Western Blotting in human chondrocyte cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Aggrecan (full length) in 10 µg of human chondrocyte cell lysate.

Target description

~270 kDa observed. The calculated molecular weight is 250 kDa However, this protein is known to be highly glycosylated and may be observed at higher molecular weights.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable