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MABN1573

Sigma-Aldrich

Anti-Synapsin-2 Antibody, clone 19.4

clone 19.4, from mouse

Synonym(s):

Synapsin II, Synapsin-2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

19.4, monoclonal

species reactivity

rat, mouse, human

technique(s)

activity assay: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

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This Item
MABN1584S2822SAB4502905
biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

19.4, monoclonal

clone

19.21, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, mouse, human

species reactivity

rat, human, mouse

species reactivity

mouse, human, rat

species reactivity

mouse, rat, human

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

-

General description

Synapsin-2 (UniProt Q63537; also known as Synapsin II) is encoded by the Syn2 gene (Gene ID 29179) in rat species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.

Specificity

Clone 19.4 reacts with both synapsin-2 spliced isoforms (IIa and IIb). Clone 19.4 does not react with any of the two synapsin-1 spliced isoforms (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).

Immunogen

Epitope: Domains A&B
Purified recombinant rat synapsin-2.

Application

Anti-Synapsin-2 Antibody, clone 19.4 is an antibody against Synapsin-2 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Enzyme Assay.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Synapsin-2 in human cerebral cortex, mouse cerebellum, and rat cerebral cortex tissue.
Western Blotting Analysis: Target detection by a representative lot was blocked by a preincubation with phage particles displaying peptides containing the SYIxN consensus sequence (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Western Blotting Analysis: A representative lot detected synapsin IIa/IIb, but not Ia/Ib, in rat brain cytosolic extracts (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Immunoprecipitation Analysis: A representative lot immunoprecipitated synapsin IIa/IIb, but not Ia/Ib, from rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Enzyme Assay Analysis: A representative lot, when added 2 hours prior to cAMP, prevented PKA-catalyzed phosphorylation of synapsin IIa/IIb in an in vitro kinase assay using rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synapsin-2 in 10 µg of rat brain tissue lysate.

Target description

~63 and 52 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    P Vaccaro et al.
    Brain research. Molecular brain research, 52(1), 1-16 (1998-02-05)
    The synapsins are a family of major neuron-specific synaptic vesicle-associated phosphoproteins which play important roles in synaptic function. In an effort to identify molecular tools which can be used to perturb the activity of the synapsins in in vitro as
    Kalpana D Acharya et al.
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