MABF213

Anti- STING, clone 41 Antibody

clone 41, 0.5 mg/mL, from rat

• Synonym(s): Stimulator of interferon genes protein, mSTING, Endoplasmic reticulum interferon stimulator, ERIS, Mediator of IRF3 activation, MMITA, Transmembrane protein 173, STING
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 41, monoclonal
• species reactivity: mouse
• concentration: 0.5 mg/mL
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_082537
• UniProt accession no.: Q3TBT3
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Sting1(72512)

Description

General description

STING, also known as mSTING, or Endoplasmic reticulum interferon stimulator, ERIS, Mediator of IRF3 activation, MMITA or Transmembrane protein 173, and encoded by the gene Tmem 173/Eris/Mita/Mpys/Sting, is a facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferons. STING acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol. Upon binding STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. STING also mediates cell death signaling via the activation of the ERK pathway. STING is found in multiple intracellular locations including the endoplasmic reticulum, plasma cell membrane, mitochondrion outer membrane, and perinuclear regions. STING is expressed in the B-cell lineage both immature and mature B cells but not plasma cells. Mutations in STING cause defects in innate immunity and compromised immune health.

Immunogen

Recombinant protein corresponding to the cytoplasmic domain of mouse STING.

Application

Flow Cytometry Analysis: 2 µg of this antibody detected STING in mouse splenocyte cells.

This Anti- STING, clone 41 Antibody is validated for use in Western Blotting and Flow Cytometry for the detection of STING.

Quality

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected STING in 10 µg of RAW264.7 cell lysate.

Target description

~42 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~38 kDa and ~48 kDa

Physical form

Format: Purified

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable