MABE1112

Anti-PRMT9 Antibody, clone 128-29-1

clone 128-29-1, from mouse

• Synonym(s): Putative protein arginine N-methyltransferase 9, Putative protein arginine N-methyltransferase 10
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 128-29-1, monoclonal
• species reactivity: human
• should not react with: mouse
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_612373
• UniProt accession no.: Q6P2P2
• target post-translational modification: unmodified
• Gene Information: human ... PRMT9(90826)

Description

General description

Putative protein arginine N-methyltransferase 9 (UniProt Q6P2P2; also known as Putative protein arginine N-methyltransferase 10) is encoded by the PRMT9 (also known as PRMT10) gene (Gene ID 90826) in human. Mammalian protein arginine methyltransferases (PRMTs) catalyze the methylation of arginine residues on histone and non-histone proteins. PRMTs are divided into three types based on their mode of activity. Type I PRMTs (PRMT1–4, PRMT6, and PRMT8) transfer up to two methyl groups on the same terminal guanidino nitrogen of arginine to form ω-NG-monomethylarginine (MMA) and ω-NG,NG-asymmetric dimethylarginine (ADMA) residues. Type II PRMTs (PRMT5 and PRMT9) transfer methyl groups on different terminal guanidino nitrogens to form ΜΜΑ and ω-NG,N′G-symmetric dimethylarginine (SDMA) residues, while type III PRMT7 transfers only one single methyl group to form MMA residues. PRMT9 is involved in alternative splicing regulation by modulating spliceosomal small nuclear ribonucleoprotein (snRNP) maturation in the cytoplasm. PRMT9 complexes with the splicing factors SF3B2 (SAP145) and SF3B4 (SAP49) and mediates SF3B2 Arg508 modification via MMA and SDMA. Similar to PRMT7, but unlike other PRMTs, PRMT9 contains a duplicated methyltransferase domain (a.a. 137-446 and 530-845), with the N-terminal MTase acting as a funtional AdoMet-binding domain and the C-terminal MTase domain serving a pseudodimer function, mimicking the homodimer formation of other PRMTs needed for subtrate processing. In addition, PRMT9 contains three tetratricopeptide repeats (TPR1-3; a.a. 25-58, 67-100, 101-134) known to mediate protein-protein interactions.

Specificity

Clone detected GST fusion containing recombinant human PRMT9 fragment a.a. 401-550, as well as endogenous human, but not mouse, PRMT9 in cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428). Epitope region is present in both spliced isoforms of human PRMT9 reported by UniProt (Q6P2P2).

Immunogen

Epitope: First MTase domain.

GST-tagged recombinant protein corresponding to the N-terminal half of human PRMT9.

Application

Anti-PRMT9 Antibody, clone 128-29-1 is an antibody against PRMT9 for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
General Post-translation Modification

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected PRMT9 in 20 µg of HeLa cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PRMT9 in human kidney, testis, and cerebellum tissue sections.
Immunocytochemistry Analysis: A representative lot detected a predominant cyotoplasmic PRMT9 localization by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed HeLa cells (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PRMT9 from HeLa cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Western Blotting Analysis: A representative lot detected GST fusion protein containing human PRMT9 a.a. 401-550 recombinant fragment, but not PRMT9 a.a. 1-199, 200-400, or 551-895 GST fusion (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Western Blotting Analysis: A representative lot detected human, but not mouse, PRMT9 using lysates from various human and mouse cell lines (Yang, Y., et al. (2015). Nat. Commun. 6:6428).

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PRMT9 in 20 µg of A431 cell lysate.

Target description

~94 kDa observed. 94.50 kDa (isoform 1) and 81.86 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable