MAB3121
Anti-Bcl-XL and BclXs Antibody, clone 7B2.5
clone 7B2.5, Chemicon®, from mouse
Synonym(s):
Anti-BCL-XL
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7B2.5, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG3
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BCL2L1(598)
Specificity
Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems (Cohen, 1991). It plays a key role in tissue and organ development, as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment (Cohen & Duke, 1992). Bcl-2 and one of its homologues, Bcl-XL, protect cells from apoptosis (Nunez & Clarke, 1994; Cory, 1995), while other homologues of Bcl-2 such as Bax, Bad and Bak have been shown to enhance apoptosis (Oltvai, 1993; Farrow, 1995; Chittenden, 1995; Kiefer, 1995). Bcl-XL has been shown to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2 (Boise, 1993; Gottschalk, 1994; Gonzalez-Garcia, 1995; Dole, 1995; Shimizu, 1995). In contrast, Bad and Bax inhibit the protective functions of Bcl-XL and Bcl-2, respectively. Although heterodimerization between Bcl-XL, Bad and Bcl-2/Bax was originally thought to be essential for the differential anti-apoptotic activity of Bcl-XL and Bcl-2 (Oltvai, 1993; Yin, 1994); other results suggest that the formation of heterodimers may not be necessary for this death-repressing activity Cheng, 1996; Gottschalk, 1996). This antibody recognizes Human Bcl-XL (Mr 29 kDa) and Bcl-Xs (Mr 21 kDa).
Immunogen
Recombinant Bcl-XS.
Application
Detect Bcl-XL & BclXs using this Anti-Bcl-XL & BclXs Antibody, clone 7B2.5 validated for use in FC, IP, WB, IH.
Flow cytometry:
Immunoprecipitation (Cheng, 1996; Gottschalk, 1996)
Immunohistochemistry (Foreman, 1996; Wrone-Smith, 1995)
Western blotting: ( 0.5 μg/mL)
Optimal working dilutions must be determined by end user.
Immunoprecipitation (Cheng, 1996; Gottschalk, 1996)
Immunohistochemistry (Foreman, 1996; Wrone-Smith, 1995)
Western blotting: ( 0.5 μg/mL)
Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
Physical form
Format: Purified
Purified immunoglobulin - Ig fraction. Liquid in borate buffer saline, pH 8.2.
Storage and Stability
Store at 2-8°C for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kaposi's sarcoma tumor cells preferentially express Bcl-xL.
Foreman, K E, et al.
The American Journal of Pathology, 149, 795-803 (1996)
Discordant expression of Bcl-x and Bcl-2 by keratinocytes in vitro and psoriatic keratinocytes in vivo.
Wrone-Smith, T, et al.
The American Journal of Pathology, 146, 1079-1088 (1995)
Programmed cell death in the immune system.
Cohen, J J
Advances in Immunology, 50, 55-85 (1991)
Apoptosis and programmed cell death in immunity.
Cohen, J J, et al.
Annual Review of Immunology, 10, 267-293 (1992)
S Cory
Annual review of immunology, 13, 513-543 (1995-01-01)
The control of cell survival is of central importance in tissues with high cell turnover such as the lymphoid system, and its disruption may be a critical step in tumorigenesis. Genes homologous to bcl-2, the oncogene implicated in human follicular
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