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Key Documents

MAB1613

Sigma-Aldrich

Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1

clone H1, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

H1, monoclonal

species reactivity

rat, human, bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_004521.1

UniProt accession no.

P33176

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KIF5B(3799)

Specificity

Reacts with the major form of heavy chain of non-neuronal KHC near the N-terminus.

Immunogen

Epitope: heavy chain, non-neuronal KHC

Application

Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1 detects level of Kinesin & has been published & validated for use in ELISA, IP, RIA, WB, IC, IH.
Immunohistochemistry on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry: paraformaldehyde fixed cells; 5-10′ RT, freshly made 4% PFA; permeabilize with 0.1-.2% triton X-100 in 3% normal serum blocking buffer

Immunoblotting: recognizes 124kDa KHC from non-neuronal samples.

Immunoprecipitation

Inhibition of kinesin mediated transport

EIA/RIA

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified
Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl.pH 7.6 with 0.1% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic.
Lippincott-Schwartz, J, et al.
The Journal of cell biology, 128, 293-306 (1995)
G Vancoillie et al.
The Journal of investigative dermatology, 114(3), 421-429 (2000-02-26)
Microtubuli play an important role in the organization of organelles and membrane traffic. They are present in melanocytic dendrites through which melanosomes are transported towards keratinocytes. Besides the actin-based motility systems, microtubuli-associated motor proteins also play a critical role in
Wen-Jun Wang et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(1), 62-69 (2013-10-12)
Previous studies identified a family of organic anion transport proteins (OATPs), many of which have C-terminal PDZ binding consensus sequences. In particular, the C-terminal four amino acids of Oatp1a1, a transporter on rat and mouse hepatocytes, comprise a consensus binding
Hongtao Chen et al.
Biophysical journal, 94(4), 1508-1520 (2007-11-06)
The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that
Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
Hirokawa, N, et al.
Cell, 56, 867-878 (1989)
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