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COA/COQ

CellASIC ONIX switching plate mammalian cells (4 chamber)

Name: CellASIC ONIX switching plate mammalian cells (4 chamber)
Brand: MM

The M04 switching plates enable continuous perfusion culture for live cell analysis. Laminar flow provides rapid & uniform solution exchange for maximum cell health. The glass coverslide bottom surface ensures the highest resolution optical viewing.

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About This Item

UNSPSC Code:

41104923

eCl@ss:

32011202

NACRES:

NB.22

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Sigma-Aldrich

D2650

Dimethyl sulfoxide

T84 Cell Line human

Millipore

CAX2-MXT20

CellASIC ONIX2 Manifold XT (Temperature Controlled)

material

PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)

manufacturer/tradename

CellASIC^® ONIX

W × L × H

85.48 mm × 127.76 mm × 14.35 mm

compatibility

for use with cells sized up to 50.0 μm

shipped in

ambient

Related Categories

Cell Imaging Instruments

CellASIC^® ONIX2 Microfluidics

CellASIC® ONIX2 Microfluidics

General description

CellASIC^® ONIX switching plate mammalian cells (4 chambers) enable continuous perfusion culture for live cell analysis. Laminar flow provides a rapid and uniform solution exchange for maximum cell health. The glass cover slide bottom surface ensures the highest resolution of optical viewing.

Application

CellASIC^® ONIX switching plate mammalian cells(4 chambers) have been used:

in live-cell imaging to study the contribution of phi-particle in the autoinhibition of dynein and the activation of dynein by dynactin
in live-cell imaging of fcSAgA binding and immunoglobulin M surface clustering by fluorescence microscopy
in multiple epidermal growth factor (EGF)pulse experiments

The CellASIC^® ONIX switching plate is suitable for use in:

time-lapse analysis of adherent cells
long-term continuous perfusion experiments (3 days typical)
solution exchange experiments (induction, inhibition, drug dosing, etc.)
automated immunostaining and on-demand fixation of live cells within the culture chamber
comparison of up to four different cell types or exposure conditions (media components) in parallel, temperature, and gas atmospheric control (temperature shift, anoxic conditions, etc.)

Features and Benefits

Compatible with CellASIC^® ONIX2 Microfluidic system and ONIX2 Manifolds
Enables perfusion-based, long-term, live-cell analysis with solution switching
The plate offers a controlled and dynamic microenvironment for 2D and 3D cell cultures

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Multivalent Soluble Antigen Arrays Exhibit High Avidity Binding and Modulation of B Cell Receptor-Mediated Signaling to Drive Efficacy against Experimental Autoimmune Encephalomyelitis.

Brittany L Hartwell et al.

Biomacromolecules, 18(6), 1893-1907 (2017-05-06)

A pressing need exists for antigen-specific immunotherapies (ASIT) that induce selective tolerance in autoimmune disease while avoiding deleterious global immunosuppression. Multivalent soluble antigen arrays (SAgAPLP:LABL), consisting of a hyaluronic acid (HA) linear polymer backbone cografted with multiple copies of autoantigen

Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated.

Kai Zhang et al.

Cell, 169(7), 1303-1314 (2017-06-13)

Cytoplasmic dynein-1 binds dynactin and cargo adaptor proteins to form a transport machine capable of long-distance processive movement along microtubules. However, it is unclear why dynein-1 moves poorly on its own or how it is activated by dynactin. Here, we

Interdependence between EGFR and Phosphatases Spatially Established by Vesicular Dynamics Generates a Growth Factor Sensing and Responding Network.

Angel Stanoev et al.

Cell systems, 7(3), 295-309 (2018-08-27)

The proto-oncogenic epidermal growth factor receptor (EGFR) is a tyrosine kinase whose sensitivity to growth factors and signal duration determines cellular behavior. We resolve how EGFR's response to epidermal growth factor (EGF) originates from dynamically established recursive interactions with spatially

Articles

Real-time imaging of adherent and non-adherent cell interactions: utility of an automated microfluidic trap platform to recapitulate in vivo cell culture microenvironment

This page describes novel microfluidic approaches to trap two different cell types in precise locations in order to image and study their interactions over time.

Primary Neuron Culture and Analysis Facilitated by the CellASIC® ONIX Microfluidic Platform

This page describes a novel, optimized microfluidic platform for long-term culture of primary neurons to monitor dynamic cellular processes in real time.

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Demonstration of Long-Term Culture and In-Plate Staining Protocols Using the Cellasic^® ONIX Microfluidic Platform

This page shows the long-term culture and in-plate staining protocols using the CellASIC ONIX Microfluidic platform.