GF102
TWEAK, human
Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis.
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
biological source
human
Quality Level
Assay
>98% (SDS-PAGE and HPLC)
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
input
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... TNFSF12(8742)
General description
Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. GF102 is a 17.0 kDa protein, comprising the TNF-like extracellular domain of TWEAK.
Product Source: E.coli
Physical form
Sterile filtered and then the protein was lyophilized from 10 mM NaP pH 7.5 + 0.1 M L-Arg.
Storage and Stability
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Freeze/thaw cycles will result in significant loss of activity. After a quick spin, reconstitute in 10 mM NaP pH 7.5 to a concentration of 1 mg/ml. This solution can be diluted into other buffer solutions or stored at -20°C. Reconstituted TWEAK should be stored at -20°C or lyophilized in working aliquots.
Analysis Note
Specific Activity: GF102 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10ng/ml.
GF102 weakly induces the death of HT29 cells when cultured in the presence of IFN-g. The ED50 for this effect is between 30-45 ng/ml.
GF102 weakly induces the death of HT29 cells when cultured in the presence of IFN-g. The ED50 for this effect is between 30-45 ng/ml.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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