CBL1358
Anti-Endoglin Antibody, Extracellular, clone MJ7/18
clone MJ7/18, Chemicon®, from rat
Synonym(s):
CD105, Ancillary TGF-beta Receptor
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clone:
MJ7/18, monoclonal
application:
FACS
IHC
IP
WB
IHC
IP
WB
species reactivity:
mouse
technique(s):
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
2
biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MJ7/18, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Eng(13805)
General description
Endoglin is the regulatory component of the TGF-beta receptor complex. CD105 is a homodimer of 90 kDa subunits and is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus, and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition, stromal cells in the connective tissue of various organs are also positive for endoglin expression. CD105 has been reported to have functions in adhesion and embryonic angiogenesis, and that MJ7/18 is a pan endothelial marker (with minimal reactivity with early hematopoetic cells). Both mouse and human CD105 exhibit >70% sequence similarity in their cytoplasmic domains with type III transforming growth factor β (TGFβ) receptor.
Specificity
Specifically recognizes mouse CD105, known as endoglin.
Immunogen
Inflamed mouse skin (Ge, AZ et al, 1994).
Application
Flow Cytometry: 1μg/ 10(e6) cells in 100 μL total volume.
Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.
Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.
Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.
Optimal working dilutions must be determined by the end user.
Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.
Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.
Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Endoglin Antibody, Extracellular, clone MJ7/18 is validated for use in FC, IP, WB, IH for the detection of Endoglin.
Physical form
Format: Purified
Protein A purified immunoglobulin presented as a liquid in 100 mM borate buffered saline, containing no preservatives.
Storage and Stability
Maintain at 2-8°C for up to 12 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL: mouse vascular endothelial cells
POSITIVE CONTROL: mouse vascular endothelial cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A Z Ge et al.
Gene, 138(1-2), 201-206 (1994-01-28)
The rat monoclonal antibody, MJ7/18, which reacts selectively with the endothelium of blood vessels in mouse was used to screen a cDNA library derived from a transformed mouse brain endothelial cell line. The sequence of a cDNA encoding the cell
Nanoparticle-mediated drug delivery to tumor vasculature suppresses metastasis.
Murphy, EA; Majeti, BK; Barnes, LA; Makale, M; Weis, SM; Lutu-Fuga, K; Wrasidlo, W; Cheresh, DA
Proceedings of the National Academy of Sciences of the USA null
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