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Key Documents

AB5820

Sigma-Aldrich

Anti-Syntaxin 1 Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... STX1A(6804)

Specificity

Recognizes Syntaxin 1A and Syntaxin 1B. The epitope does not share homology with any other known proteins.

Immunogen

GST fusion protein corresponding to a cytoplasmic part of rat Syntaxin 1A (Accession number P32851) (Bennet et al. 1992; Inoue & Akagawa 1992).

Application

Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
This Anti-Syntaxin 1 Antibody is validated for use in WB, IH for the detection of Syntaxin 1.
Western blot: 1:700-1:1,250 (0.8-1.5 g/mL) using ECL on rat brain membranes.

Immunohistochemistry: AB5820 is directed against an intracellular epitope. Thus, a procedure including permeabilization of cells with 0.2% Triton X 100 is recommended. It is recommended that you start with a working concentration of 30 μg/mL (1:33).

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Inoue et al.
Biochemical and biophysical research communications, 187(2), 1144-1150 (1992-09-16)
We have already cloned the cDNA for the HPC-1 antigen, a neuron-specific protein antigen from the rat brain. Here we report the molecular cloning of the bovine HPC-1 antigen homologue, and much strong sequence conservation between rat and bovine. By
Adam D Bachstetter et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(14), 6143-6153 (2013-04-05)
Neuropathology after traumatic brain injury (TBI) is the result of both the immediate impact injury and secondary injury mechanisms. Unresolved post-traumatic glial activation is a secondary injury mechanism that contributes to a chronic state of neuroinflammation in both animal models
Sushma Dagar et al.
PloS one, 9(3), e90250-e90250 (2014-03-07)
Deafferentation is known to cause significant changes in the postsynaptic neurons in the central nervous system. Loss of photoreceptors, for instance, results in remarkable morphological and physiological changes in bipolar cells and horizontal cells. Retinal ganglion cells (RGCs), which send
Suleman Hussain et al.
Frontiers in molecular neuroscience, 9, 10-10 (2016-02-24)
Syntaxins are a family of membrane-integrated proteins that are instrumental in exocytosis of vesicles. Syntaxin-1 is an essential component of the presynaptic exocytotic fusion machinery in the brain and interacts with several other proteins. Syntaxin-1 forms a four-helical bundle complex
M K Bennett et al.
Science (New York, N.Y.), 257(5067), 255-259 (1992-07-10)
Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Two 35-kilodalton proteins (p35 or syntaxins) were identified that interact

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