AB5609
Anti-GABA A Receptor α1 Antibody, cytoplasmic loop
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clone:
polyclonal
application:
IP
WB
WB
species reactivity:
rat
technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
citations:
3
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GABRA1(2554)
Specificity
GABAA receptor alpha1 subunit, cytoplasmic loop. The apparent molecular mass of 51 kDa in rat brain membrane fractions. The antibody shows no reactivity in the forebrain of alpha1 knockout mice by Western blot.
Immunogen
Epitope: cytoplasmic loop
Fusion protein from the cytoplasmic loop of rat GABAA receptor alpha1 subunit.
Application
Anti-GABA A Receptor α1 Antibody, cytoplasmic loop is an antibody against GABA A Receptor α1 for use in IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blot: 1:1,000
Immunopreciptation: The antibody will immunoprecipitate 59% of all GABA A receptors in dodecysulfate extracts of rat forebrains (150 μL) using 15 μg of antibody.
Optimal working dilutions must be determined by the end user.
Immunopreciptation: The antibody will immunoprecipitate 59% of all GABA A receptors in dodecysulfate extracts of rat forebrains (150 μL) using 15 μg of antibody.
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol. Approximate concentration is 0.15 mg/mL.
Storage and Stability
Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Subunit composition and quantitative importance of hetero-oligomeric receptors: GABAA receptors containing alpha6 subunits.
Jechlinger, M, et al.
The Journal of Neuroscience, 18, 2449-2457 (1998)
Stephanie M Miller et al.
Journal of neurochemistry, 139(3), 471-484 (2016-10-25)
Seizures are a common manifestation of hypoxic-ischaemic brain injury in the neonate. In status epilepticus models alterations to GABAA R subunit expression have been suggested to contribute to (i) abnormal development of the GABAergic system, (ii) why seizures become self-sustaining
The synaptic proteome during development and plasticity of the mouse visual cortex.
Dahlhaus, M; Li, KW; van der Schors, RC; Saiepour, MH; van Nierop, P; Heimel, JA; Hermans et al.
Molecular and Cellular Proteomics null
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