AB3120
Anti-Angiopoietin-1 Antibody, NT
Chemicon®, from rabbit
Synonym(s):
Ang-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ANGPT1(284)
General description
The remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development. Angiopoietin-1 (mouse and human Ang-1; 498aa with 98% identity) is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic dvevelopment, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496aa with 85% identity) may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology.
Specificity
Recognizes mouse Angiopoietin-1 (Ang-1). The immunogenic peptide sequence is 90% (18/20 aa) conserved in human Ang-1, 95% I pig and bovine, and has no significant sequence homology with Ang-2. Antibody crossreactivity in other species is not established.
Immunogen
20 amino acid peptide sequence near the amino-terminus of mouse Ang-1.
Epitope: N-terminus
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Angiopoietin-1 Antibody, N-terminus is validated for use in ELISA, WB for the detection of Angiopoietin-1.
Western blot: 1-10 ug/mL using ECL. Expected size of Ang-1 is ~55kDa.
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well (Catalog Number AG635).
Immunohistochemistry: Not tested. It is recommended that the antibody be used at 2-20 ug/ml in formaldehyde fixed tissue.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well (Catalog Number AG635).
Immunohistochemistry: Not tested. It is recommended that the antibody be used at 2-20 ug/ml in formaldehyde fixed tissue.
Optimal working dilutions must be determined by end user.
Target description
55 kDa
Physical form
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Fetal heart
Fetal heart
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sertoli Cell-Initiated Testicular Innate Immune Response through Toll-Like Receptor-3 Activation Is Negatively Regulated by Tyro3, Axl, and Mer Receptors.
Sun B, Qi N, Shang T, Wu H, Deng T, Han D
Endocrinology null
C Suri et al.
Cell, 87(7), 1171-1180 (1996-12-27)
Vascular endothelial growth factor (VEGF), which acts via members of a family of endothelial-specific receptor tyrosine kinases, is the only factor that has been shown definitively to play a role in the formation of the embryonic vasculature. Only one other
Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice.
Makinde TO, Agrawal DK
American Journal of Respiratory Cell and Molecular Biology null
Sunil K Chauhan et al.
Arteriosclerosis, thrombosis, and vascular biology, 35(7), 1606-1615 (2015-05-23)
In angiogenesis, circulating mononuclear cells are recruited to vascular lesions; however, the underlying mechanisms are poorly understood. Here, we characterize the functional role of protein tyrosine kinase 7 (PTK7)-expressing CD11b(+) mononuclear cells in vitro and in vivo using a mouse
Susan M Dallabrida et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22(8), 3010-3023 (2008-05-27)
Angiopoietins were thought to be endothelial cell-specific via the tie2 receptor. We showed that angiopoietin-1 (ang1) also interacts with integrins on cardiac myocytes (CMs) to increase survival. Because ang1 monomers bind and activate integrins (not tie2), we determined their function
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