8.52418

Fmoc-Asp(OBno)-OH

Novabiochem^®

• Synonym(s): Fmoc-Asp(OBno)-OH
• Empirical Formula (Hill Notation): C₃₂H₄₃NO₆
• Molecular Weight: 537.69
• UNSPSC Code: 12352209

Properties

• Quality Level: 200
• product line: Novabiochem^®
• form: powder
• reaction suitability: reaction type: Fmoc solid-phase peptide synthesis
• manufacturer/tradename: Novabiochem^®
• application(s): peptide synthesis
• functional group: carboxylic acid
• storage temp.: −20°C (−15°C to −25°C)

Description

General description

An excellent derivative for minimizing aspartimide formation during Fmoc SPPS, including those containing the Asp-Gly sequence. The bulky OBno protecting group offers considerably more protection against the formation of aspartimide-related by-products than the commonly used OtBu and OMpe group.^,

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Overcoming Aspartimide Formation in Fmoc SPPS

Literature references:
^[1] R. Behrendt, et al. (2015) J. Pept. Sci., 21, 680.
^[2] R. Behrendt, et al. (2016) J. Pept. Sci., 22, 92.

Application

Recently, Fmoc-Asp(OBno)-OH has been used in the synthesis of the mini-protein Omomyc, which has been shown to repress MYC-dependent gene transcription.,

Analysis Note

Color (visual): white to beige
Appearance of substance (visual): powder, chunks or crystals
Identity (IR): passes test
Purity (TLC (018A)): ≥ 95 %
Enantiomeric purity: ≥ 99.5 % (a/a)
Assay (HPLC, area%): ≥ 97.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable