553571

Anti-Ras (31-43) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-Ras GDP/GTP Binding Protein
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat, bovine, human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAB2A(5862)

Description

General description

Immunoaffinity rabbit polyclonal antibody. Recognizes all forms of wild type Ras and the mutant G12V Ras protein.

Recognizes all forms of wild type Ras, including the mutant G12V Ras protein.

This Anti-Ras (31-43) Rabbit pAb is validated for use in Immunoblotting for the detection of Ras (31-43).

Immunogen

a synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Rat brain tissue

Other Notes

By immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.

Hardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418,Academic Press, San Diego, CA.
Sawada, M., et al. 1989. Nucleic Acid Res.17, 8867.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable