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Supelco

Purospher® STAR Si (5 µm)

L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column

Synonym(s):

Purospher® STAR SI (5µm) Hibar® RT 250-4.6

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About This Item

UNSPSC Code:
41115700

material

stainless steel column

Agency

suitable for USP L3

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

parameter

400 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

Silica bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

hydrophilic interaction (HILIC)
normal phase
supercritical fluid (SFC)

storage temp.

no temp limit

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General description

Purospher® STAR Si endcapped, 5 µm HPLC columns offers highest separation efficiency for normal-phase chromatography of low molecular weight compounds soluble in organic solvents.Purospher® STAR Si, 5 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The recommended guard column is Purospher® STAR Si (5 µm) LiChroCART® 4-4 part number 1.50249.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.51487.0001 can be used.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex samples allowing significant solvent savings and improved sensitivity.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, carotenoids, oils, fat soluble vitamins, lipids, and fatty acids.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Use with Normal-phase or HILIC conditions (needs to be flushed to HILIC mobile phase)
  • Good suitability for LC-MS applications

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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