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1.01865

Millipore

Readybag® Half Fraser Broth with Supplements

acc. ISO 11290, 62 g, irradiated, For the primary selective enrichment of Listeria sp.

Synonym(s):

Demi Fraser broth, Fraser broth primary enrichment, Fraser Broth, Fraser Listeria selective enrichment Broth

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74

Agency

APHA
ISO 11133
ISO 11290

Quality Level

200

form

medium granules (dehydrated (DCM))

manufacturer/tradename

ReadyBag®

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

solubility

55 g/L

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Listeria spp.

General description

Half FRASER (Demi FRASER) broth has high nutrient content and the large buffer capacity that creates optimum growth conditions for Listeria. Lithium chloride, nalidixic acid, and acriflavine hydrochloride largely inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate enables the detection of the β-D-glucosidase activity by the formation of a black complex of esculetin with iron (III) ions. However, this reaction is not exclusive to Listeria sp. and hence every primary and secondary enrichment in FRASER broth has to be sub-cultured on selective plating media.

Application

Half FRASER (Demi FRASER) broth with supplements is used for the primary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Protocols

Listeria: a Survivor

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

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