04-1540

Anti-PICH Antibody, clone 142-26-3

clone 142-26-3, from mouse

• Synonym(s): excision repair cross-complementing rodent repair deficiency, complementation group 6-like, excision repair cross-complementing rodent repair deficiency complementation group 6 - like, Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-de
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 142-26-3, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_060139
• UniProt accession no.: Q2NKX8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ERCC6L(54821)

Description

General description

Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome.

Specificity

This antibody recognizes Plk1-interacting checkpoint helicase.

Immunogen

Epitope: Unknown

Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase.

Application

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like.

Quality

Evaluated by Western Blot in HeLa-S3 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.

Target description

~160 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa-S3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable