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Key Documents

L030000

Sigma-Aldrich

ETT Activator

0.25 M

Synonym(s):

5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution

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About This Item

MDL number:
UNSPSC Code:
41116105
PubChem Substance ID:
NACRES:
NA.21

type

for DNA synthesis

product line

Proligo Reagents

storage condition

dry at room temperature

concentration

32-35 g/L (ETT content, titration)

impurities

≤30 ppm water content (Karl Fischer)

color

clear

SMILES string

CCSc1nnn[nH]1

InChI

1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)

InChI key

GONFBOIJNUKKST-UHFFFAOYSA-N

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Preparation Note

Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)

Other Notes

Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

35.6 °F

Flash Point(C)

2.0 °C


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