mAbs Characterization & Quality Control

The key for ensuring drug safety and efficacy

HomeIntegrated OfferingsMonoclonal Antibodies (mAbs)Characterization and Quality ControlPost-Translational Modification Analysis

Characterization and quality control of monoclonal antibodies (mAbs) is essential to ensure their efficacy and safety. Analytical techniques, like HPLC and mass spectrometry, are vital to assess various properties, such as aggregation, charge variants, and post-translational modifications (PTMs). Our extensive selection of products supports the detailed analysis and evaluation of mAbs to maintain quality and effectiveness.

Characterization and Quality Control

Intact Mass Analysis

Peptide Mapping

Post-Translational Modification Analysis

Aggregate Analysis

Titer Determination

Microbiological Testing

Post-Translational Modification Analysis of Monoclonal Antibodies

Post-translational modification analysis of monoclonal antibodies (mAbs) assesses product consistency, efficacy, and safety. To identify and quantify PTMs in mAbs, mass spectrometry and chromatography are commonly used. High-purity solvents are used to prevent contamination and ensure consistency. HPLC columns separate and quantify different components of the mAb sample. Analytical reagents are used for characterization and detection of mAbs. Glycobiology profiling tools are used to analyze the carbohydrate structures of mAbs.

We offer all the tools you need to monitor and ensure the consistency, quality, and safety of mAbs across various stages of the mAb production workflow.

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HPLC Columns

Explore RPC, SEC, IEC, HILIC, and IMAC columns.

Large, brown solvent bottle with Supelco® branded label is opened by a lab worker wearing blue nitrile gloves. Only the hands and lower arms of the worker are shown. One small brown solvent bottle and two small white bottles can be seen in the background.

High-Purity Solvents

Use our highest grade of solvents in mAb production to minimize the risk of impurities.

Dark-haired female lab worker wearing safety goggles, white lab coat and blue nitrile gloves prepares a solution of reagents. Glass vials, glass beakers and a computer screen are visible in the background.

Analytical Reagents

Count on our high-quality reagents for analytical characterization in various stages of mAb production.

Female scientist wearing white lab coat, blue nitrile gloves, and safety goggles pipetting a protein solution into a centrifugal tube. Male scientist wearing same protection clothing looking into microscope is visible in the background.

Glycobiology Profiling Tools

Find glycosidases and glycobiology reagents and kits to support glycobiology profiling of mAbs.

Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.
Large Molecule HPLC
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Released N-Linked Glycan Analysis Workflow of Adalimumab
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
Learn about the advantages of these columns compared to commercial FPP columns for large molecule separation.
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