Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Appendix 1, extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, reduce the need for stringent washing procedures, and extend the life of the chromatographic medium.

Sample extraction procedures and the selection of buffers, additives, and detergents are determined largely by the source of the material, the stability of the target molecule, the chromatographic techniques that will be employed and the intended use of the product. These subjects are dealt with in general terms in the Strategies for Protein Purification Handbook and more specifically according to target molecule in the handbooks Affinity Chromatography, Vol. 1: Antibodies, 18103746 and Vol. 2: Tagged Proteins, 18114275 available from GE.

Sample stability

In the majority of cases, biological activity needs to be retained after purification. Retaining the activity of the target molecule is also an advantage when following the progress of the purification, since detection of the target molecule often relies on its biological activity. Denaturation of sample components often leads to precipitation or enhanced nonspecific adsorption, both of which will impair column function. Hence there are many advantages to checking the stability limits of the sample and working within these limits during purification.

Proteins generally contain a high degree of tertiary structure, kept together by van der Waals’ forces, ionic and hydrophobic interactions, and hydrogen bonding. Any conditions capable of destabilizing these forces can cause denaturation and/or precipitation. By contrast, peptides contain a low degree of tertiary structure. Their native state is dominated by secondary structures, stabilized mainly by hydrogen bonding. For this reason, peptides tolerate a much wider range of conditions than proteins. This basic difference in native structures is also reflected in that proteins are not easily renatured, while peptides often renature spontaneously.

It is advisable to perform some stability tests before beginning to develop a purification protocol. The list below shows examples of such testing:

• Test pH stability in steps of one pH unit between pH 2.0 and pH 9.0.
• Test salt stability with 0 to 2 M NaCl and 0 to 2 M (NH4)2SO4 in steps of 500 mM.
• Test the stability towards acetonitrile and methanol in 10% steps between 0% and 50%.
• Test the temperature stability in 10 °C steps from 4 °C to 40 °C.
• Test the stability and occurrence of proteolytic activity by leaving an aliquot of the sample at room temperature overnight. Centrifuge each sample and measure activity and UV absorbance at 280 nm in the supernatant.

Sample clarification

Centrifugation and filtration are standard laboratory techniques for sample clarification and

are used routinely when handling small samples.

It is highly recommended to centrifuge and filter samples immediately before chromatographic purification.

Centrifugation

Centrifugation removes lipids and particulate matter, such as cell debris. If the sample is still not clear after centrifugation, use filter paper or a 5 µM filter as a first step and one of the filters below as a second-step filter.

• For small sample volumes or proteins that adsorb to filters, centrifuge at 10 000 × g for 15 min.
• For cell lysates, centrifuge at 40 000 to 50 000 × g for 30 min.
• Serum samples can be filtered through glass wool after centrifugation to remove remaining lipids.

Filtration

Filtration removes particulate matter. Whatman™ syringe filters, which give the least amount of nonspecific binding of proteins, are composed of cellulose acetate (CA), regenerated cellulose (RA), or polyvinylidene fluoride (PVDF) (Table A1.1).

Table A1.1 Whatman syringe filters for filtration of samples

Filter pore size (µM)	Up to sample volume (mL)	Whatman syringe filter1	Membrane
0.8	100	Puradisc FP 30	CA
0.45	1	Puradisc 4	PVDF
0.45	10	Puradisc 13	PVDF
0.45	100	Puradisc 25	PVDF
0.45	10	SPARTAN™ 13	RC
0.45	100	SPARTAN 30	RC
0.45	100	Puradisc FP 30	CA
0.2	1	Puradisc 4	PVDF
0.2	10	Puradisc 13	PVDF
0.2	100	Puradisc 2	PVDF
0.2	10	SPARTAN 13	RC
0.2	100	SPARTAN 30	RC
0.2	100	Puradisc FP 30	CA

¹The number indicates the diameter (mm) of the syringe filter.
For sample preparation before chromatography, select a filter pore size in relation to the bead size of the chromatographic medium (Table A1.2).

Table A1.2 Selecting a sample filter based on the bead size of the chromatographic medium used

Nominal pore size of filter (µM)	Particle size of chromatographic medium (µM)
1.0	90 and upwards
0.45	30 or 34
0.22	3, 10, 15 or when extra clean samples or sterile filtration is required

Check the recovery of the target protein in a test run. Some proteins adsorb nonspecifically to filter surfaces.

Desalting

Desalting columns are suitable for any sample volume and will rapidly remove LMW contaminants in a single step at the same time as transferring the sample into the correct buffer conditions. Centrifugation and/or filtration of the sample before desalting is still recommended. Detailed procedures for buffer exchange and desalting are given in the section Buffer exchange and desalting later in this appendix.

At laboratory scale, when samples are reasonably clean after filtration or centrifugation, the buffer exchange and desalting step can be avoided. For AC or HIC, it might be sufficient to adjust the pH of the sample. For IEX, it might be sufficient to dilute the sample to reduce the ionic strength.

Specific sample preparation steps

Specific sample preparation steps might be required if the crude sample is known to contain contamininants such as lipids, lipoproteins, or phenol red that might build up on a column. Gross impurities, such as bulk protein, should be removed before any chromatographic step.

Fractional precipitation

Fractional precipitation is occasionally used at laboratory scale to remove gross impurities from the sample. Precipitation techniques separate fractions by the principle of differential solubility. Because proteins differ in their degree of hydrophobicity, increased salt concentrations can enhance hydrophobic interactions between the proteins and cause precipitation. Fractional precipitation can be applied to remove gross impurities in three different ways, as shown in Figure A1.1.

Figure A1.1. Three ways to use precipitation.

Precipitation techniques can be affected by temperature, pH, and sample concentration. These parameters should be controlled to ensure reproducible results.

Examples of precipitation agents are reviewed in Table A1.3. The most common precipitation method using ammonium sulfate is described in more detail.

Table A1.3 Examples of precipitation techniques

Precipitation agent	Typical conditions for use	Sample type	Comment
Ammonium sulfate	As described below.	> 1 mg/mL proteins especially immuno-globulins.	Stabilizes proteins, no denaturation, supernatant can go directly to HIC. Helps to reduce lipid content.
Dextran sulfate	Add 0.04 mL 10% dextran sulfate and 1 mL of 1 M CaCl2 per mL sample, mix 15 min, centrifuge 10 000 × g, discard pellet.	Samples with high levels of lipoprotein, e.g., ascites.	Precipitates lipoprotein.
Polyvinylpyrrolidine	Add 3% (w/v), stir 4 h, centrifuge 17 000 × g, discard pellet.	Samples with high levels of lipoprotein,e.g., ascites.	Alternative to dextran sulfate.
Polyethylene glycol (PEG, Mr > 4000)	Up to 20% w/v.	Plasma proteins.	No denaturation, supernatant goes directly to IEX or AC, complete removal might be difficult. Stabilizes proteins
Acetone (cold)	Up to 80% v/v at ± 0°C. Collect pellet after centrifugation at full speed in a microcentrifuge.		Can denature protein irreversibly. Useful for peptide precipitation or concentration of sample for electrophoresis.
Polyethyleneimine	0.1% w/v.		Precipitates aggregated nucleoproteins.
Protamine sulphate	1% w/v.		Precipitates aggregated nucleoproteins.
Streptomycin sulfate	1% w/v.		Precipitation of nucleic acids.
Caprylic acid	(X/15) g where X = volume of sample.	Antibody concentration should be > 1 mg/mL.	Precipitates bulk of proteins from sera or ascites, leaving immunoglobulins in solution

Details taken from: Scopes R. K., Protein Purification, Principles and Practice, Springer, (1994), J. C. Janson and L. Rydén, Protein Purification, Principles, High Resolution Methods and Applications, second ed. Wiley Inc, (1998). Personal communications.

Ammonium sulfate precipitation

Some proteins can be damaged by ammonium sulfate. Take care when adding crystalline ammonium sulfate; high local concentrations can cause contamination of the precipitate with unwanted proteins.

For routine, reproducible purification, precipitation with ammonium sulfate should be avoided in favor of chromatography.

In general, precipitation is rarely effective for protein concentrations below 1 mg/mL.

Solutions needed for precipitation:

Saturated ammonium sulfate solution (add 100 g ammonium sulfate to 100 mL distilled water, stir to dissolve).

1 M Tris-HCl, pH 8.0.

Buffer for first purification step.

1. Filter (0.45 µM) or centrifuge the sample (10 000 × g at 4 °C).
2. Add 1 part 1 M Tris-HCl, pH 8.0 to 10 parts sample volume to maintain pH.
3. Stir gently. Add ammonium sulfate solution, drop by drop. Add up to 50% saturation1. Stir for 1 h.
4. Centrifuge 20 min at 10 000 × g.
5. Remove supernatant. Wash the pellet twice by resuspension in an equal volume of ammonium sulfate solution of the same concentration (i.e. a solution that will not redissolve the precipitated protein or cause further precipitation). Centrifuge again.
6. Dissolve pellet in a small volume of the buffer to be used for the next step.
7. Ammonium sulfate is removed during clarification/buffer exchange steps with Sephadex G-25, using desalting columns (see Buffer exchange and desalting later in this appendix).

¹ The percent saturation can be adjusted either to precipitate a target molecule or to precipitate contaminants.

The quantity of ammonium sulfate required to reach a given degree of saturation varies according to temperature. Table A1.4 shows the quantities required at 20 °C.

Table A1.4 Quantities of ammonium sulfate required to reach given degrees of saturation at 20 °C

Final percent saturation to be obtained
	20	25	30	35	40	45	50	55	60	65	70	75	80	85	90	95	100
Starting percent saturation	Amount of ammonium sulfate to add (gram) per liter of solution at 20 °C
0	113	144	176	208	242	277	314	351	390	430	472	516	561	608	657	708	761
5	85	115	146	179	212	246	282	319	358	397	439	481	526	572	621	671	723
10	57	86	117	149	182	216	251	287	325	364	405	447	491	537	584	634	685
15	28	58	88	119	151	185	219	255	293	331	371	413	456	501	548	596	647
20	0	29	59	89	121	154	188	223	260	298	337	378	421	465	511	559	609
25		0	29	60	91	123	157	191	228	265	304	344	386	429	475	522	571
30			0	30	61	92	125	160	195	232	270	309	351	393	438	485	533
35				0	30	62	94	128	163	199	236	275	316	358	402	447	495
40					0	31	63	96	130	166	202	241	281	322	365	410	457
45						0	31	64	98	132	169	206	245	286	329	373	419
50							0	32	65	99	135	172	210	250	292	335	381
55								0	33	66	101	138	175	215	256	298	343
60									0	33	67	103	140	179	219	261	305
65										0	34	69	105	143	183	224	267
70											0	34	70	107	146	186	228
75												0	35	72	110	149	190
80													0	36	73	112	152
85														0	37	75	114
90															0	37	76
95																0	38

Resolubilization of protein precipitates

Many proteins are easily resolubilized in a small amount of the buffer to be used in the next chromatographic step. However, a denaturing agent may be required for less soluble proteins. Specific conditions will depend upon the specific protein. These agents must always be removed to allow complete refolding of the protein and to maximize recovery of mass and activity. A chromatographic step often removes a denaturant during purification. Table A1.5 gives examples of common denaturing agents.

Table A1.5 Denaturing agents used for resolubilization of relatively insoluble proteins

Denaturing agent	Typical conditions for use (molar, M)	Removal/comment
Urea	2 to 8	Remove using Sephadex G-25
Guanidine hydrochloride	3 to 6	Remove using Sephadex G-25

Details taken from: Scopes R. K., Protein Purification, Principles and Practice, Springer, (1994), J. C. Janson and L. Rydén, Protein Purification, Principles, High Resolution Methods and Applications, second ed. Wiley Inc, (1998) and other sources.

Buffer exchange and desalting

Dialysis is frequently mentioned in the literature as a technique to remove salt or other small molecules and to exchange the buffer composition of a sample. However, dialysis is generally a very slow technique, requiring large volumes of buffer. There is also a risk of losing material during handling or as a result of proteolytic breakdown or nonspecific binding to the dialysis membranes. A simpler and much faster technique is to use a desalting column, packed with Sephadex G-25, to perform a group separation between HMW and LMW substances. Proteins are separated from salts and other small molecules.

In a fast, single step, the sample is desalted, transferred into a new buffer and LMW materials are removed.

Desalting columns are used not only to remove LMW contaminants, such as salt, but also for buffer exchange before or after different chromatographic steps and for the rapid removal of reagents to terminate a reaction.

Sample volumes up to 30% of the total volume of the desalting column can be processed. Sample concentration does not influence the separation as long as the concentration of proteins does not exceed 70 mg/mL when using normal aqueous buffers. The sample should be fully dissolved. Centrifuge or filter to remove particulate material.

For small sample volumes, it is possible to dilute the sample with the start buffer that is to be used for chromatographic purification, but cell debris and particulate matter must still be removed.

Volatile buffers such as 100 mM ammonium acetate or 100 mM ammonium hydrogen carbonate can be used if it is necessary to avoid the presence of NaCl.

Figure A1.2 shows a typical buffer exchange and desalting separation. The process can be monitored by following changes in UV absorption and conductivity.

Figure A1.2. Buffer exchange of mouse plasma (10 mL) on HiPrep 26/10 Desalting.

Table A1.6 Examples of desalting and buffer exchange columns

Column	Sample volume (mL)	Sample elution volume (mL)
PD MiniTrap G-25	0.2 to 0.5	0.1 to 0.5
PD-10 (gravity feed column)	1.0 to 2.5	3.5
HiTrap® Desalting, 5 mL	0.25 to 1.5	1.0 to 2.0
HiPrep 26/10 Desalting	2.5 to 15	7.5 to 20

To desalt larger sample volumes:

• Connect up to five HiTrap^® Desalting 5 mL columns in series to increase the sample volume capacity, for example, two columns: sample volume 3 mL, five columns: sample volume 7.5 mL.
• Connect up to four HiPrep 26/10 Desalting columns in series to increase the sample volume capacity, for example two columns: sample volume 30 mL, four columns: sample volume 60 mL. Even with four columns in series, the sample can be processed in 20 to 30 min, at room temperature, in aqueous buffers.

Instructions are supplied with each column. Desalting and buffer exchange can take less than 5 min per sample with greater than 95% recovery for most proteins.

Manual desalting with HiTrap^® Desalting 5 mL using a syringe

1. Fill the syringe with buffer. Remove the stop plug. To avoid introducing air into the column, connect the column “drop to drop” to the syringe (via the adapter provided).
2. Remove the snap-off end.
3. Wash the column with 25 mL buffer at 5 mL/min to remove completely the 20% ethanol (supplied as storage buffer). If air is trapped in the column, wash with degassed buffer until the air disappears. Air bubbles introduced onto the column by accident during sample application do not influence the separation.
4. Apply the sample (0.25 to 1.5 mL) using a 2 to 5 mL syringe at a flow rate between 1 to 10 mL/min. Discard the liquid eluted from the column.
5. If the sample volume is less than 1.5 mL, change to buffer and proceed with the injection until a total of 1.5 mL has been eluted. Discard the eluted liquid.
6. Elute the protein with the appropriate volume selected from Table A1.7 and collect the desalted protein.

Note: 5 mL/min corresponds to approximately 120 drops/min when using a HiTrap^® 5 mL column. A simple peristaltic pump or a chromatography system can also be used for the desalting procedure.

The maximum recommended sample volume is 1.5 mL. Table A1.7 for the effect of reducing the sample volume applied to the column.

Table A1.7 Recommended sample and elution volumes using a syringe

Sample load (mL)	Add buffer (mL)	Elute and collect (mL)	Yield (%)	Remaining salt (%)	Dilution Factor
0.25	1.25	1.0	> 95	0.0	4.0
0.50	1.0	1.5	> 95	< 0.1	3.0
1.00	0.5	2.0	> 95	< 0.2	2.0
1.50	0	2.0	> 95	< 0.2	1.3

Removal of lipoproteins

Lipoproteins and other lipid material can rapidly clog chromatography columns and it is advisable to remove them before beginning purification. Precipitation agents such as dextran sulfate and polyvinylpyrrolidine, described under Fractional precipitation above, are recommended to remove high levels of lipoproteins from samples such as ascitic fluid.

Centrifuge samples when performing precipitation to avoid the risk of nonspecific binding of the target molecule to a filter.

Samples such as serum can be filtered through glass wool to remove remaining lipids.

Removal of phenol red

Phenol red is frequently used at laboratory scale as a pH indicator in cell culture. Although not directly interfering with purification, phenol red binds to certain purification media and should be removed as early as possible to avoid the risk of contamination. It is known to bind to AIEX media at pH > 7.0.

Use a desalting column to simultaneously remove phenol red (a low molecular weight molecule) and transfer sample to the correct buffer conditions for further purification, as described under Buffer exchange and desalting earlier in this appendix.

Removal of LMW contaminants

If samples contain a high level of LMW contaminants, use a desalting column before the first chromatographic purification step, as described under Buffer exchange and desalting earlier in this appendix.