HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 (Acetone-Acetonitrile Mobile Phase, ELSD Detection)
CONDITIONS
column
Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)
mobile phase
[A] acetone; [B] acetonitrile; (60:40, A:B)
flow rate
1.5 mL/min
pressure
914 psi (63 bar)
column temp.
30 °C
detector
ELSD
injection
5 μL
sample
5% soybean oil in acetone (w/v)
Description
Analysis Note
Soybean oil was analyzed as 5% diluted sample in acetone (w/v)
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany