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HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53931-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

47768

Ergocalciferol (D2)

analytical standard

47773

Phylloquinone (K1)

analytical standard

47783

(±)-α-Tocopherol

analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)

mobile phase

methanol

flow rate

1.0 mL/min

pressure

1653 psi (114 bar)

column temp.

30 °C

detector

UV, 280 nm

injection

5 μL

sample

0.4 mg/mL tocopherol acetate, alpha-tocopherol and K1, 0.04 mg/mL D2 and D3, all in ethanol

Description

Analysis Note

Analysis was conducted using standard solution, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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