Skip to Content
Merck
  • Acetonitrile adduct formation as a sensitive means for simple alcohol detection by LC-MS.

Acetonitrile adduct formation as a sensitive means for simple alcohol detection by LC-MS.

Journal of the American Society for Mass Spectrometry (2014-09-25)
Roy Bogseth, Eric Edgcomb, Christopher M Jones, Edward K Chess, Peifeng Hu
ABSTRACT

Simple alcohols formed protonated acetonitrile adducts containing up to two acetonitrile molecules when analyzed by ESI or APCI in the presence of acetonitrile in the solvent. These acetonitrile adducts underwent dissociation to form a nitrilium ion, also referred to as the substitution ion. Diols and triols behaved differently. In ESI, they formed only one acetonitrile adduct containing one acetonitrile. The S ion was not observed in ESI and was only weakly observed from the dissociation of the (M + ACN + H)(+) ion. On the other hand, the S ion was abundantly formed from the diols in APCI. This formation of acetonitrile adducts and substitution ion from simple alcohols/diols offers an opportunity to detect simple alcohols/diols sensitively by LC-MS interfaced by ESI or APCI. The utility of this chemistry was demonstrated in a method developed for the quantification of cyclohexanol in rat plasma by monitoring the CID-induced fragmentation from the S ion to a fragment ion.

MATERIALS
Product Number
Brand
Product Description

Propylene glycol, European Pharmacopoeia (EP) Reference Standard
Supelco
Glycerol, analytical standard
Sigma-Aldrich
Glycerol, puriss., anhydrous, 99.0-101.0% (alkalimetric)
Sigma-Aldrich
Glycerol solution, puriss., meets analytical specification of Ph. Eur., BP, 84-88%
Sigma-Aldrich
Glycerol, ReagentPlus®, ≥99.0% (GC)
Sigma-Aldrich
Glycerol, ACS reagent, ≥99.5%
USP
Propylene glycol, United States Pharmacopeia (USP) Reference Standard
USP
Glycerin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
1-Butanol, 99.9%
Sigma-Aldrich
Glycerol, puriss. p.a., ACS reagent, anhydrous, dist., ≥99.5% (GC)
Sigma-Aldrich
1-Butanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
1-Butanol, ACS reagent, ≥99.4%
Sigma-Aldrich
1-Butanol, suitable for HPLC, ≥99.7%
Sigma-Aldrich
2-Propanol, for molecular biology, BioReagent, ≥99.5%
Sigma-Aldrich
Glycerol, for molecular biology, ≥99.0%
Sigma-Aldrich
Glycerol, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
Sigma-Aldrich
Glycerin, meets USP testing specifications
Sigma-Aldrich
1-Butanol, for molecular biology, ≥99%
Sigma-Aldrich
Propylene Glycol, meets USP testing specifications
Sigma-Aldrich
Glycerol, BioXtra, ≥99% (GC)
Sigma-Aldrich
Glycerol, ≥99.5%
Sigma-Aldrich
Glycerol solution, 83.5-89.5% (T)
Sigma-Aldrich
1-Butanol, anhydrous, 99.8%
Supelco
1,2-Propanediol, analytical standard
Sigma-Aldrich
Propylene glycol, ≥99.5% (GC), FCC, FG
Sigma-Aldrich
1,2-Propanediol, meets analytical specification of Ph. Eur., BP, USP, ≥99.5%
Sigma-Aldrich
1,2-Propanediol, puriss. p.a., ACS reagent, ≥99.5% (GC)
Sigma-Aldrich
1,2-Propanediol, tested according to Ph. Eur.
Sigma-Aldrich
1,2-Propanediol, ReagentPlus®, 99%
Sigma-Aldrich
Glycerol, FCC, FG