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A simple colorimetric method for the estimation of plasma biliverdin.

Clinica chimica acta; international journal of clinical chemistry (1978-04-17)
T R Tickner, J M Gutteridge
PMID417882
ABSTRACT

A new colorimetric method for the assay of biliverdin in biological fluids is described. The method, based upon the reaction of biliverdin with barbituric acid, offers improved sensitivity and selectivity when compared to direct spectrophotometric measurements. Using this method biliverdinaemia was observed in two patients with obstructive jaundice of malignant origin.

MATERIALS
Product Number
Brand
Product Description
A0278
Sigma-Aldrich
L-Ascorbic acid, reagent grade
95210
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)
33034
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)