- Growth and Purification of Vaccinia Virus Stocks for MPM Imaging.
Growth and Purification of Vaccinia Virus Stocks for MPM Imaging.
Methods in molecular biology (Clifton, N.J.) (2019-06-27)
Glennys V Reynoso, John P Shannon, Jeffrey L Americo, James Gibbs, Heather D Hickman
PMID31240685
ABSTRACT
This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.