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Membrane Immunodetection Devices

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A professional in a lab coat and purple gloves working with a Membrane Immunodetection device in a well-equipped laboratory, surrounded by various laboratory equipment and supplies, highlighting the precision and care involved in immunodetection processes.

Western blotting immunodetection involves the use of antibodies to detect target protein. Blotted membranes containing transferred proteins are blocked, followed by antibody incubation and detection using chemiluminescence, colorimetric, or fluorescent methods. Membrane immunodetection devices facilitate the processing of Western blots by simplifying workflows and accelerating detection times. 

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The Snap I.D. 2.0 protein detection system, showcasing a sleek white base with two blue adjustment knobs and a viewing screen, alongside designated areas for sample placement, embodying modern protein analysis technology

SNAP i.d.® 2.0 Protein Detection System

The SNAP i.d.® 2.0 Protein Detection System is a unique, vacuum-driven system that pulls reagents through the membrane, increasing membrane contact while eliminating dependence on diffusion or agitation. This improvement in three-dimensional reagent distribution decreases the length of time required for immunodetection. While traditional Western blotting requires 4 to 24 hours for the blocking, antibody incubation, and washing steps, the SNAP i.d.® 2.0 protocol takes only 30 minutes with no loss of signal intensity or reduction in blot quality. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP i.d.® 2.0 System.

Related Resources

  • Protocol: Protein Blotting Handbook

    The seventh edition of the Protein Blotting Handbook offers comprehensive guidance on optimizing antibody concentrations and reducing background interference, along with expanded data and protocols for fluorescence detection. The handbook represents the collective experience of application scientists and technical service specialists.

  • Article: 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System

    The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

  • Brochure: Rethink Western Blotting

    Access to our Western blotting expertise is easy—flip to our troubleshooting section at the end of this brochure, or learn more.

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Related Product Categories
Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
Transfer Buffers & Reagents
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.
Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Related Applications
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
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