H4290

Hydroxyphenyl fluorescein solution

5 mM in DMF

• Synonym(s): Hydroxyphenyl fluorescein, 2-[6-(4′-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid, HPF
• Empirical Formula (Hill Notation): C₂₆H₁₆O₆
• Molecular Weight: 424.40
• EC Number: 200-679-5
• UNSPSC Code: 12352108
• NACRES: NA.32

Properties

• Quality Level: 200
• concentration: 5 mM in DMF
• fluorescence: λ_ex 490 nm; λ_em 515 nm
• storage temp.: 2-8°C

Description

General description

Hydroxyphenyl fluorescein solution, also known as HPF is a fluorescent probe, which upon oxidation by the hydroxyl radical (HO) returns a brilliant green fluorescence (HPF-ox). Increased HPF signals in antibiotic-exposed bacterial cells are explained by fluorescence associated with increased cell size, not by ROS levels. In cells incubated with the HPF probe and exposed to ampicillin and norfloxacin with HPF fluorescence correlated linearly.

Application

Fluorescent reagent for the detection of highly reactive oxygen species (hROS). Fluorescence intensity increases after the immediate reaction with hROS such as hydroxyl radical and peroxynitrite. Since HPF does not react with nitric oxide, superoxide and hydrogen peroxide, it can differentially detect peroxynitrite. HPF is resistant to light-induced autoxidation.

Hydroxyphenyl fluorescein solution is used to detect highly reactive oxygen species (hROS). It is a suitable probe to detect cellular death caused by bacterial antibiotics.

Features and Benefits

The following are the benefits of Hydroxyphenyl fluorescein solution:

• HPF is resistant to light-induced autooxidation.
• Hypochlorite(-OCl) does not react with HPF.
• HPF does not react with nitric oxide, superoxide, and hydrogen peroxide. 
• HPF has low reactivity with HOCl.

Safety Information

• Pictograms: GHS02,GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H226,H312 + H332,H319,H360D
• Precautionary Statements: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 135.5 °F - (Dimethylformamide)
• Flash Point(C): 57.5 °C - (Dimethylformamide)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves