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CWDS-MFB630

Sigma-Aldrich

ColorWheel® Antibody-Ready mFluor Blue 630 Dye

greener alternative

Synonym(s):

mFluor Blue 630

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About This Item

UNSPSC Code:
12352203

antibody form

purified antibody

Quality Level

antibody product type

primary antibodies

product line

ColorWheel®

form

lyophilized

purified by

gel-filtration chromatography

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

compatibility

for use with ColorWheel® Dye-ready Antibodies (Required, Sold Separately)

greener alternative category

storage temp.

2-8°C

target post-translational modification

unmodified

General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

ColorWheel® Antibody-Ready mFluor Blue 630 Dye is designed for use with ColorWheel® Dye-Ready antibodies in Flow cytometry.

Application

Flow Cytometry: Excitation: 470 nm, Emission: 634 nm

Compatibility

Requires ColorWheel® Antibody (Sold Separately)

Target description

mFluor Blue 630 is a water-soluble dye and the protein conjugates prepared with this dye are well suited for flow cytometry detections. It offers a large Stokes shift with an excitation at 470 nm and emission at 634 nm. When compared to R-Phycoerythrin (RPE), it offers greater photostability. It displays an extinction coefficient of 49,000 cm-1 M-1.

Physical form

CWDS-MFB630-25UL Set Components

CWD-MFB630-25UL - ColorWheel® Antibody-Ready mFluor Blue 630 Dye

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel® Finishing Buffer

Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
mFluor is a trademark of AAT Bioquest

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Experience simplicity in your flow cytometry workflow with the 3-Step ColorWheel® Flow Cytometry Reagent Preparation protocol. With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel® flow cytometry antibodies and dyes.

View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining.

Related Content

Unlock freedom in your flow cytometry assay design with ColorWheel® flow cytometry antibodies and dyes. The ColorWheel® flow cytometry portfolio was designed with simplicity and flexibility in mind to help streamline your flow cytometry workflow without compromising on quality.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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