CWA-1143

Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb

• Synonym(s): Leukocyte surface antigen CD47;Integrin-associated protein
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MEM-122, monoclonal
• product line: ColorWheel^®
• form: lyophilized
• mol wt: calculated mol wt 35.19 kDa
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): nonhuman primates
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• technique(s): flow cytometry: suitable
• isotype: IgM
• epitope sequence: Extracellular domain
• Protein ID accession no.: Q08722
• UniProt accession no.: A0A0D9R1W9
• compatibility: for use with ColorWheel^® Dyes (Required, (Sold Separately))
• greener alternative category: , Aligned
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: Monkey ... CD47(103228373)

Description

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone MEM-122 is a mouse monoclonal antibody that detects CD47. It targets an epitope within the extracellular domain.

Immunogen

COS-7 cells

Application

Quality Control Testing

Evaluated by Flow Cytometry in A431 cells.

Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Leukocyte surface antigen CD47 (UniProt: A0A0D9R1W9; also known as Integrin-associated protein, CD47) is encoded by the CD47 gene (Gene ID: 103228373) in Monkey. CD47 is a multi-pass transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form. It is widely expressed on all hematopoietic cells and most other cell types. The ligands of CD47 include SIRP , thrombospondin-1 (TSP-1), and integrins including vβ3 and 2β1. It plays a role in vβ3-mediated cell adhesion and Ca2+ influx, as well as in platelet activation via interaction with thrombospondin-1. In addition, CD47 modulates peripheral mononuclear (PMN) neutrophil migration across endothelial and epithelial monolayers. The rate of fMLP-stimulated PMN migration is shown to be decreased by CD47 functional blockage upon neutralizing antibody treatment, which can be partially reversed by PI3K inhibition. Following fMLP stimulation and after PMN transmigration, secondary granules-derived CD47 molecules are shown to accumulate on plasma membrane, resulting in increased cell surface CD47 immunoreactivity. Overexpression of CD47 is reported on the surface of many types of cancer cells where it forms a signaling complex with signal-regulatory protein (SIRP ), enabling the escape of these cancer cells from macrophage-mediated phagocytosis. (Ref.: Zhang, W., et al. (2020). Front. Immunol. 11; 18).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable