Z374873
PCR microtubes with attached caps
capacity 0.2 mL
About This Item
Recommended Products
material
colorless cap
colorless tube
conical bottom
flat cap
polypropylene
polypropylene cap
polypropylene tube
description
Autoclavable
sterility
non-sterile
feature
RNase and DNase free
autoclavable at 121 °C
graduations: no
Push fit
packaging
pack of 250 ea
case of 4 packs
technique(s)
PCR: suitable
capacity
0.2 mL
color
clear
suitability
suitable for (PCR applications)
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General description
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
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