Z374873
PCR microtubes with attached caps
capacity 0.2 mL
Synonym(s):
PCR Tubes
About This Item
Recommended Products
material
colorless cap
colorless tube
conical bottom
flat cap
polypropylene
polypropylene cap
polypropylene tube
description
Autoclavable
sterility
non-sterile
feature
RNase and DNase free
autoclavable at 121 °C
graduations: no
Push fit
packaging
pack of 250 ea
case of 4 packs
technique(s)
PCR: suitable
capacity
0.2 mL
color
clear
suitability
suitable for (PCR applications)
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General description
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
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